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DOT Urine
Drug Testing

The Department of Transportation (DOT) urine drug testing is a federally mandated drug testing program for safety-sensitive employees, such as commercial truck drivers, pilots, and railroad workers, who are subject to drug and alcohol testing under DOT regulations.

Ensuring the safety of your workplace

The DOT urine drug testing program is designed to ensure the safety of the traveling public and prevent accidents caused by drug or alcohol impairment.

The DOT urine drug test screens for five commonly abused drugs: marijuana, cocaine, amphetamines, opiates, and phencyclidine (PCP). The test is a laboratory-based test that analyzes a urine sample collected under strict chain of custody procedures to ensure the integrity of the sample.

The DOT urine drug testing program requires that all safety-sensitive employees undergo pre-employment drug testing, as well as random, post-accident, reasonable suspicion, and return-to-duty drug testing. Employers must use DOT-approved drug testing laboratories and follow strict testing procedures to ensure the accuracy of the test results.

If an employee tests positive for drugs or alcohol, they may be subject to disciplinary action, including termination of employment. Employees who test positive may also be required to complete a substance abuse treatment program and pass a return-to-duty drug test before being allowed to return to safety-sensitive duties.

It is important for employers and employees subject to DOT regulations to understand the requirements of the DOT urine drug testing program and comply with all testing procedures to ensure the safety of the traveling public and avoid penalties for non-compliance.